The existing biological methods for the evaluation of estrogen have been complicated? inaccurate, furthermore, npn-specific in procedures.
The recent advances in the chromatography has brought the chemical methods for the evaluation of the chemical estrogen i.d. estrone, estradiol and estriol by fractional assesment.
As a part of the research project in this deportment related to the various tests for the evaluation - of the functions of hypophysis, thyroid gland, ovary and-adrenal cortex, the evaluation of the urinary estrogen obtained from the females with regular menstrual cycles (7 cases) and with the menstrual irreguarity (11 cases) by fraction technique, were carried out.
The results are as follows:
1) In the females with regularity of menstrual cycles, the presence of the urinary estrgen was elicited throughout cycles, the highest levels of the same substance were maintained at, the ovulation time and the lowest, being during the menstruation, average levels were at 91 gamma/24 hrs.
2) In the cases with secondary amenorrhea the levels of estrogen were rather low in 3 cases out of 5, the remaining showing normal levels. In primary amenorrhea, all 2 cases revealed at lower contents.
3) In cases with irregularity, all 4 cases were observed to be maintained with normal levels.
4) The fractions of estradiol, estrone and estriol were found that the lowest levels were maintained by estradiol, the highest, by estriol.
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